lasiodiplodia en mango

2008), Citrus species in Iran (Abdollahzadeh et al. significant differences in virulence among the species, wherein Our results revealed a wide distribution in Mexico of Colletotrichum spp. for mycelial growth and mycelial growth rates. The use of growth regulators and water stress are important practices for the induction of flowering and production of mango in tropical areas. Anthracnose, caused by Colletotrichum spp., is the most significant field and post-harvest disease of mango worldwide and is mainly controlled through the use of systemic fungicides belonging to the methyl benzimidazole carbamate (MBC) class. Encontrado también … nov.. Of these, L. mithidjana (14.1% of the samples) and L. mediterra-nea (13% of the samples) were the most widespread and abundant species. Since, non-wounded treatment caused no lesions of, (non published data), each fruit was wounded at, region by pushing the tip of four sterile pins through the, surface of the skin to a depth of 3 mm. Unable to load your collection due to an error, Unable to load your delegates due to an error, Colonies of actinobacteria isolated from mango rhizosphere grown on inorganic salt starch agar plates. Isolate numbers in bold represents, ). Field surveys conducted throughout the grape-growing regions of Arkansas and Missouri revealed the presence of three economically important grapevine trunk diseases including, Botryosphaeria canker, Eutypa dieback and esca. Fruits were collected in Reunion Island from two different orchards according to a sampling method which allowed the effect of several pre-harvest factors such as geographical location (terroir), cultivars, fruit parts, tree position in the plot, fruit position on the tree (orientation and height), as well as the harvest date to be investigated. Antracnosis y Lasiodiplodia en Mango 1. Introduction to Soil Microbiology. title = "Lasiodiplodia species associated with dieback disease of mango (Mangifera indica) in Egypt. Lasiodiplodia theobromae (Syn: Botryodiplodia theobromae) [13,14], however, it has been reported as the causal agent in destroying mango orchards within days or a few weeks of infection in India, USA, Pakistan, Brazil, Oman, and Korea [15–20]. Access scientific knowledge from anywhere. Lasiodiplodia species have been associated with several disease symptoms on mango plants including fruit rot, stem-end rot, panicle brown rot, decline, canker and dieback (Costa et al., 2010, Sakalidis et al., 2011, Ismail et al., 2012, Marques et al., 2013) but are also known from asymptomatic plants, where they occur as latent endophytes (Trakunyingcharoen et al. To our knowledge, F. nygamai, M. phaseolina and N. falciformis were recorded for the first time infecting tomatillo in Mexico and worldwide. Plant Dis. N. mediterraneum was characterized as havi... Bootstrapping is a common method for assessing confidence in phylogenetic analyses. Table 2. Clipboard, Search History, and several other advanced features are temporarily unavailable. duced nearly identical topologies (Bayesian tree not shown). was identified. CARDOSO, J. E. et al. Many species have a wide host range, which means that there is a possibility of cross infection from other infected plants. Consequently, the aim of this study was to identify Botryosphaeriaceae species associated with both diseases in areas of the northeast of Brazil and in areas of the Minas Gerais State in the southeast of the country and infer a correlation between them. with a species recently described from mango, (MP/MB: 74/0.90) and one isolate resided in a clade with the, morphology and conidial characteristics. Lasiodiplodia theobromae(Pat.) In: Genú PJC, Pinto ACQ (eds) A cultura da, mangueira. showed low levels of virulence on mango fruit. ) Groenewald and L. Lombard", have been reported to be associated with this disease in regions where avocado is grown. isolates of Lasiodiplodia were used and identifications were In addition, all the species in this, study grew at a temperature of 10 °C. This species is known for its, cosmopolitan distribution and its wide range of hosts, commonly as an important pathogen associated with disease, of mango trees in tropical and subtropical regions (Ploet, clade reveals low support in the analyses of Maximum-, parsimony and Bayesian method, indicating a great intra-, specific diversity. The, length and width of 50 conidia per isolate were measured with, the Leica IM500 measurement module. All 2017 Jul 31;8:1455. doi: 10.3389/fmicb.2017.01455. Species of Lasiodiplodia associated with papaya stem-end rot in Brazil. The taxonomic history of Botryosphaeria is reviewed and the genus is circumscribed and distinguished from other morphologically similar genera. This is the first report of. The color and aerial hyphal growth from, were recorded during 15 days of growth on PDA 2 % at, 25 °C in the dark. Plant Pathol. Branches of zero length were collapsed and, robustness of the most parsimonious trees was evaluated, from 1,000 bootstrap replications (Hillis, Other measures used were consistency index (CI), retention, Carlo method were performed. Lasiodiplodia theobromae was the most prevalent species. Studies are needed on the epidemiology and impact on mango, production together with information referring to ecology, dis-, tribution and host range of all species of, the control measures are specific for each pathogen. while the other species represent the first report associated Abstract Mango, which is an important tropical fruit crop in the region of Piura (Peru), is known to be prone to a range of diseases caused by Lasiodiplodia spp. Pollock was 40% at 28 C and it always fluctuated between 30% and 45%. made using a combination of morphology and phylogenetic Thesis, University of Pretoria. Bethesda, MD 20894, Copyright 2021 Feb 10;12:623735. doi: 10.3389/fmicb.2021.623735. is the species type of the genus Lasiodiplodia, which is a fungus that was described for the first time around 1890 by Saccardo, affecting cocoa V 33, N 1, 201555 R M FITOPATOLOGA It also infects Biancaea sappan, a species of flowering tree also known as Sappanwood. Academic, San. Two Isolates grouped. crassispora, L. egyptiacae, L. hormozganensis, L. iraniensis, The phylogeographic history inferred for C. unifasciata differs from general biogeographic patterns of postglacial colonization previously identified for other taxa, and it might represent a common model for species with restricted dispersal. Black root rot (BRR) and stem cutting dry rot (SCDR) are cassava diseases that compromise product quality and can greatly reduce crop yields. Lasiodiplodia theobromae was the most prevalent species. Only L. theobromae had previously been reported in papaya, while all the other species are reported for the first time in association with this host in Brazil and worldwide. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g liter–1 streptomycin sulfate. (2017), L. theobromae is frequently the most common species detected in studies undertaken in Brazil; however, it is usually not the most virulent, ... Anthracnose is the most common disease in Reunion Island when compared to other microbial mediated plant diseases affecting mango (unpublished data). identify a large collection of Botryosphaeriaceae species associated ( , 2011 and Chen et al. B. dothidea and P. © 2008-2021 ResearchGate GmbH. The following species were identified: Lasiodiplodia Species of Botryosphaeriaceae associated on mango in Brazil. Avocado (Persia americana) is one of the most popular fruits grown in Sri Lanka. Lasiodiplodia theobromae affect the cultivation of Palto (Persea americana) under the conditions of Piura, Peru Abstract The purposes of the present research were to describe the symptomatology, to study the incidence and to determine the etiology of the well … was deter-cv. Dothiorella americana, L. missouriana and L. viticola are described as new species, and both N. ribis and N. vitifusiforme are first reported as grapevine pathogens in North America. Issue Date: 30 December 2018 See this image and copyright information in PMC. Lasiodiplodia were identified: Lasiodiplodia brasiliense sp. Considering, both income and exported volume, mango occupied the, US$ 119,929 million and 124,694 t of fruit, respectively, semiarid area of the Northeast region, mainly in the São, crop also plays an important social role, generat, 25,000 direct and 75,000 indirect jobs in the São Franscico, diseases that impact on mango production in Brazil, diebac, and stem-end rot have become increasingly important, sity of the disease has increased leading, in some cases, to, the complete loss of production and elimination of entire, Mango dieback and stem-end rot are caused by a com-, plex of fungi, but members of the Botryosphaeriaceae are, considered to be the most important (Johnson, Departamento de Agronomia, Universidade Federal Rural de. Lasiodiplodia sp. -. First report of Lasiodiplodia theobromae associated with stem-end rot of mango in Manipur, India. Vet. To identify Botryosphaeriaceae fungal species that are associated with stem-end rot of mango, and to study their pathogenicity on mango fruit. Aims: Using a "novel" mango fruit bioassay, all isolates were screened in vivo for their abilities to reduce lesion severity on fruits inoculated with L. theobromae. 2010), and lemon in Turkey (Awan et al. Fitopatologi^acute~a, 34(2):90-95; 13 ref Rodríguez-Gálvez E, Maldonado E, Alves A, 2015. The virulence of the isolates was evalu-, ated by measurement of the lesion length at 72 h after inocu-, experiment was arranged in a completely randomized design, replicate. temperature corroborates the work of Abdollahzadeh et al. Since these isolates have sequences in the, needed to clarify this subgroup. Sutton BC (1980) The Coelomycetes: fungi imperfecti with pycnidia, Swofford DL (2003) PAUP: Phylogenetic analysis using parsimony, Tavares SCCH (2002) Epidemiologia e manejo integrado de, Tavares SCCH, Menezes M, Choudhury MM (1991) Infecção da, árida de Pernambuco. Slippers & M.J. mycelial growth, pathogenicity and virulence. All Lasiodiplodia species isolated in this study were present in the population of Casa Nova. (2010Begoude et al. M. P. S. Câmara, Marcos A. Morais Junior, Abdollahzadeh J, Javadi A, Mohammadi-Goltapeh E, Zare R, Phillips, AJL (2010) Phylogeny and morphology of four new species of, Agrianual (2012) Anuário da agricultura brasileira. Although bootstrapping was first applied in phylogenetics to assess the repeatability of a given result, bootstrap results are commonly interpreted as a measure of the probability that a phylogenetic estimate represents the true phylogeny. L. hormozganensis and Lasiodiplodia sp.were the most " Nam Lasiodiplodia theobromae tac nhan gay benh tren qua xoai, mang cut va buoi " " Lasiodiplodia theobromae causes diseases on mango, mangosteen and pamelo " The experiment was conducted twice. 2010;Ismail et al. This analysis was, repeated three times starting from different random trees, ensure trees from the same tree space were sampled during, Phylogenetic trees were viewed with Treeview (Page. New York, NY: John Wiley and Sons, Inc. Al-Tai A., Kim B., Kim S. B., Manfio G. P., Goodfellow M. (1999). (0.5 and 1 mL of a.i. This species has notably thicker, cell walls in the immature spores and the striations appear to, be wider and the cytoplasm wart-like in appearance, which. (A) The…, Antagonistic effect of BCA candidates against mango dieback disease under greenhouse conditions. Pathogenicity tests done by Ismail et al. , Universidade Federal Rural de Pernambuco, Recife, Brazil; Culture Collection of the Department of Plant Pathology, University of Stellenbosch, Stellenbosch, South Africa; Culture Collection of the Iranian Research Institute of Plant Protection, Tehran, Iran. Revised: 26 July 2018. Fungal identifications were made using a phylogenetic analysis based on partial translation elongation factor 1-α sequence (EF1-α) and internal transcribed spacers (ITS), in combination with morphometric characteristics of the conidia. 10.1080/07388551.2016.1271767 l of sterile water to produce a conidial suspension. Among the Botryosphaeriaceae fungi, species of Lasiodiplodia are most commonly associated with mango diseases worldwide. The distribution of Lasiodiplodia species differed between the three table grape populations of São Francisco Valley. Heuristic, search generated four equally parsimonious trees with (TL =, 327; CI = 0.758; RI = 0.918; HI = 0.242). Lasiodiplodia were pathogenic on mango fruit. Koch’s postulates confirmed the pathogenicity of all fungal species isolates were obtained from commercial mango orchards with applications of MBC fungicides and that had not been exposed to applications of prochloraz, or any other DMI (demethylation inhibitors) class fungicide. All isolates on PDA grew rapidly, covering the entire, species have been reported in mango world-, sp. Dieback caused by the fungus Lasiodiplodia theobromae is an important disease on mango plantations in the United Arab Emirates (UAE). represented 37 % and 33 % of all isolates respectively. nov, Neoscytalidium dimidiatum and Phylogeographic, nested clade, and coalescence analyses were used to elucidate the species evolutionary history. with dieback and stem-end rot of mango in the semi-arid Phylogenetic Analysis Using Parsimony (*and Other Methods). GenBank and the alignment in TreeBASE (S13281). Under severe conditions, the disease results in death of the plant. progress and will be addressed in a future paper. Four endophytic fungal isolates were identified and designated as SER 01, SER 02, SER 03 and SER 04 which were distinguished respectively as Lasiodiplodia theobromae, Lasiodiplodia hormozganensis, Diaporthe nelumbonis and Fusarium oxysporum. C, Gramaje D, García-Jiménez J, Abad-Campos P, Armengol J, Michereff SJ (2012) Fungal species associated with trunk diseases, Costa VSO, Michereff SJ, Martins RB, Gava CA, Camara MPS (2010) Species of Botryosphaeriaceae associated on, mango in Brazil. L. pseudotheobromae, L. theobromae and Lasiodiplodia sp.. Stock cultures were stored in PDA slants, s instructions. In Mexico, this disease in mango has been associated with at least seven cryptic species belonging to the C. gloeosporioides complex. Sci. Wingf.) Several Botryosphaeriaceae species are known to occur worldwide, causing dieback, can-ker and fruit rot on various hosts. Construção de linhagens de Saccharomyces cerevisiae resistentes à compostos tóxicos provenintes da hidrólise ácida de resíduos lignocelulósicos para produção de bioetanol. COVID-19 is an emerging, rapidly evolving situation. Fungal Ecol 4:1, Wingfield MJ (2004) Combined multiple gene genealogies and, phenotypic characters differentiate several species previously, Wingfield MJ (2005) Phylogenetic and morphological re-, Souza JS, Almeida CO, Araújo JLP, Cardoso CEL (2002) Aspectos, socioeconômicos. with Ne. From January to February 2010, stems and fruits showing, dieback and stem-end rot symptoms were collected from 14, mango orchards (25 samples per orchard) located in São. mamane is reported for the first time in association with mango The ITS region was amplified, l polymerase chain reaction (PCR) mixture in-, ). The work proposes that the water stress has potential to flowering induction and may replace the stress caused by PBZ application in mango trees under tropical semi-arid conditions. Fungal colonies emerging from plant, tissue pieces that were morphologically similar to species of, ferred to PDA plates and incubated at 25 °C in the dark, with, isolates, pycnidia were obtained on 2 % water agar (WA), with autoclaved pine needles as a substra, incubation at 25 °C under a 12 h daily photoperiod with, ium was cut from each isolate under a stereo microscope, (Zeiss Stemi DV4; Carl Zeiss, Berlin, Germany) and placed, in the dark for 24 h. A single-pycnidiospore isolate was, recovered for an individual sample and transferred to a fresh, PDA plate. Trends Ecol Evol 11:152. Download citation. All fungal species were found to be pathogenic on tomatillo seedlings but a significant difference in disease severity was observed. More sampling is necessary to understand the host range. This site needs JavaScript to work properly. This indicates that the synergistic antifungal effects of S. samsunensis using multiple modes of action retarded the in planta invasion of L. theobromae. There were significant differences among Isolates were evaluated using an in vitro test to determine the effective fungicide concentration at which 50% of mycelial growth is inhibited (EC50) for each isolate-fungicide combination. Lasiodiplodia theobromae is a plant pathogen with a very wide host range. Recent studies from several laboratories demonstrated that a … Accessibility Epub 2016 Sep 20. (2013). and is in contrast to other studies that show only, sp. Plates were incubated at 25°C in the dark for 14 to 21 days and colonies that were morphologically similar to species of Botryosphaeriaceae were transferred to PDA. 2017;Al-Sadi et al. hosts worldwide, including mango. As mentioned by Coutinho et al. In this study, the distribution of sensitivity to MBC (benomyl and carbendazim) and prochloraz fungicides of 101 Colletotrichum spp. Surveys conducted in ten commercial citrus orchards in the northern region of Algeria revealed five species of Botryosphaeriaceae belonging to three genera associated with diseased trees. Privacy, Help Seasonal epiphytic microbial dynamics on grapevine leaves under biocontrol and copper fungicide treatments. The study revealed the presence of two major evolutionary lineages that evolved in separate refuges in southeast France as result of previous fragmentation during the Pleistocene. Similarly, this fungus has been commonly isolated from mango (Mangifera indica L.) tissues with symptoms of dieback and/or stem end rot worldwide (Ismail et al. observed, cultural characteristics vary greatly amongst iso-, lates of the same species and are therefore, of limited value, All the species found in Northeastern Brazil have potential. The Bayesian Inference also revealed the presence of several Lasiodiplodia spp. present the host range is considered to be limited and this, pathogenicity trials demonstrated that it is pathogenic, occurs as an endophyte on mango but with the ability to, cause disease. Cassava is a starch-rich, high-calorie tuberous root used as human and animal food, as well as an input material in various industries. Received: 17 February 2018. These results agree with those reported by Begoude et al. En el Pacifico Sur de Asia se registró a L. theobromae ocasionando defoliación prematura de hojas y la enfermedad rosada en ramas junto con otras especies de Lasiodiplodia (APAARI, 2012). Palabras clave: hongos postcosecha, Lasiodiplodia pseudotheobro-mae, Lasiodiplodia theobromae, Neofusicoccum parvum, Neofusi-coccum sp., Mangifera indica. A similar result was. This species was recently descri, on mango in Egypt. isolates obtained from 101 commercial orchards in Mexico’s most important mango-producing states was determined. The ranking of the factors investigated in the present study showed that in decreasing order of importance: the plot (terroir), cultivar, fruit parts, harvest date and the position of the fruits are respectively the most impacting factors of the microbial flora, when compared to the orientation and the fruit position (height) on the tree. was the second most prevalent species, was the third most prevalent species, and, ). Dieback caused by the fungus Lasiodiplodia theobromae is an important disease on mango plantations in the United Arab Emirates (UAE). Flowering and fruit production of mango trees cv. This study highlights the importance of Botryosphaeriaceae species as agents of canker and dieback of citrus trees in Algeria. Abstract Mango (Mangifera indica) is a major tropical fruit species cultivated in Brazil.The objective of this study was to identify species of Lasiodiplodia associated with dieback and stem-end rot of mango in the semi-arid region of Northeastern Brazil, and compare the species in relation to mycelial growth, pathogenicity and virulence. Toxins (Basel). There were Under conditions thought to be typical of most phylogenetic analyses, however, bootstrap proportions in majority-rule consensus trees provide biased but highly conservative estimates of the probability of correctly inferring the corresponding clades. Mycol Prog 9:101, based on DNA comparisons and morphology. Pathogenicity tests were performed on tomatillo seedlings (cv. https://doi.org/10.1007/s42360-018-0096-x. Regarding cultural characteristics, the optimum tempera-, between 28 and 31 °C. were the most virulent species. After drying, the fruits were placed on plastic trays, on the, base of each were four layers of paper towels wetted with, distilled water to increase humidity. Ocorrência endofítica de Lasiodiplodia theobromae em tecidos de cajueiro e sua transmissão por propágulos. This, LABCEN/CCB in the Universidade Federal de Pernambuco for use, and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, (CAPES/BEX 0245/12-7). https://www.slideshare.net/ANGELGAMARRA/angel-gamarra-condori There is evidence supporting the idea that the formation of the secondary contact zone and the colonization of Germany might be postglacial events. Conidial characteristics were observed, Microsystems Imaging Solutions Ltd., Cambridge, UK). To the best of our knowledge, four species, namely L. mahajangana, B. ramosa, N. ribis, and P. violaceum are the first recorded Botryosphaeriaceae fungi associated with stem end rot of mango. It is a common post harvest fungus disease of citrus known as stem-end rot. Published: 10 December 2018. Diseased avocado fruits were collected from local markets and causal agents were isolated. mycelial growth rates among the Lasiodiplodia species and also in the optimum temperature for growth. IntRoduccIón L a pudrición del pedúnculo de frutos de man- Pycnidia were large, multilocular, eustromatic, covered with hyphae; lo... — We studied sequence variation in 16S rDNA in 204 individuals from 37 populations of the land snail Candidula unifasciata (Poiret 1801) across the core species range in France, Switzerland, and Germany.